Pacific Biosciences

Pacific Biosciences

A biotechnology company founded in 2004 that develops and manufactures systems for gene sequencing and some novel real time biological observation

Single Molecular Real-Time (SMRT) sequencing employs a specialized flow cell with many thousands of individual picolitre wells with transparent bottoms -- zero-mode waveguides (ZMW). The polymerase is fixed to the bottom of the well and allows the DNA strand to progress through the ZMW. As a result, the system can focus on a single molecular. SMRT sequencing allows for real-time imaging of fluorescently tagged nucleotides that are synthesized along individual DNA template molecules. The sequencing reaction ends when the template and polymerase dissociate. The average read length from the PacBio instrument is approximately 2 kb, and some reads may be over 20 kb. Longer reads are especially useful for de novo assemblies of novel genomes that can span many more repeats and bases.

Highly repetitive elements found in both eukaryotic and prokaryotic genomes pose a challenge for genome assembly and make the detailed study of repetitive sequences difficult. Long-read sequencing delivers reads in excess of several or dozens of kilobases (kbs), which can span complex or repetitive regions with a single continuous read, allowing for the resolution of these large structural features. Besides considerably longer and highly accurate DNA sequences from individual unamplified molecules, it can also exhibit where methylated bases occur, thereby providing functional information about DNA methyltransferases encoded by the genome.

Timeline

People

Name
Role
LinkedIn

Aaron Holiday

Investor

Aaron Talebi

Employee

Aiyesha Ma

Employee

Alex Gottschalk

Employee

Alex Hutcheson

Employee

Alvin Lin

Investor

Aniket Deshpande

Employee

ankit master

Employee

Arianna Gianola

Employee

Asif Khalak

Employee

Ben Lerch

Employee

Ben Voiles

Employee

Bryan Shieh

Employee

Casey Marshall

Employee

Chris Kurdziel

Investor

CJ Halabi

Investor

Craig Hamasaki

Employee

Dana Underwood

Employee

Darisha Jhutty

Employee

Davian Contreras

Employee

David Evans

Employee

David Hsu

Employee

David Singer

Investor

Dimitris Iliopoulos

Employee

Donald Gray

Employee

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Further reading

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Documentaries, videos and podcasts

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Companies

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News

Title
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Description
BioSpace
June 10, 2020
BioSpace
Pacific Biosciences of California, Inc. (NASDAQ: PACB), a leading provider of high-quality sequencing of genomes, transcriptomes and epigenomes, today announced that Michael Hunkapiller, Ph.D. is retiring as Chief Executive Officer and President by the end of the year. Dr. Hunkapiller plans to remain on the Board of Directors. The company als

References

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