A biosensor is an analytical device, used for the detection of a chemical substance, that combines a biological component with a physicochemical detector.

The sensitive biological element, e.g. tissue, microorganisms, organelles, cell receptors, enzymes, antibodies, nucleic acids, etc., is a biologically derived material or biomimetic component that interacts with, binds with, or recognizes the analyte under study. The biologically sensitive elements can also be created by biological engineering. The transducer or the detector element, which transforms one signal into another one, works in a physicochemical way: optical, piezoelectric, electrochemical, electrochemiluminescence etc., resulting from the interaction of the analyte with the biological element, to easily measure and quantify. The biosensor reader device connects with the associated electronics or signal processors that are primarily responsible for the display of the results in a user-friendly way. This sometimes accounts for the most expensive part of the sensor device, however it is possible to generate a user friendly display that includes transducer and sensitive element (holographic sensor). The readers are usually custom-designed and manufactured to suit the different working principles of biosensors.

If the biosensor is to be used for invasive monitoring in clinical situations, the probe must be tiny and biocompatible, having no toxic or antigenic effects. If it is to be used in fermenters it should be sterilisable. This is preferably performed by autoclaving but no biosensor enzymes can presently withstand such drastic wet-heat treatment. In either case, the biosensor should not be prone to fouling or proteolysis.

There are three so-called 'generations' of biosensors; First generation biosensors where the normal product of the reaction diffuses to the transducer and causes the electrical response, second generation biosensors which involve specific 'mediators' between the reaction and the transducer in order to generate improved response, and third generation biosensors where the reaction itself causes the response and no product or mediator diffusion is directly involved.

Biosensors can be categorized as per biological element or transduction element used. Biological elements typically used are enzymes, micro-organisms, antibodies, biological tissue, organelles etc. The method of transduction depends on type of physicochemical change as a result of sensing event. Primarily biosensors based on transducer element are mass based, electrochemical biosensors and optical biosensors.

• Mass based Biosensors include Magnetoelectric, Piezoelectric (QCM-Quartz crystal microbalance, SAW ) etc.

• Electrochemical Biosensors include potentiometric sensors, Amperometric sensors, Impedimetric sensors, conductometric sensors etc.




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