Patent 8236499 was granted and assigned to Pacific Biosciences on August, 2012 by the United States Patent and Trademark Office.
Provided are methods and compositions for the production of linear single-stranded nucleic acids, which can be used as templates in high-throughput sequencing systems. Also provided are methods and compositions for the production of closed single-stranded nucleic acid loops, which can be used as templates in high-throughput sequencing systems.