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US Patent 7620500 Optimization of crossover points for directed evolution

Patent 7620500 was granted and assigned to Maxygen, Inc. on November, 2009 by the United States Patent and Trademark Office.

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Patent
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Patent attributes

Current Assignee
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Maxygen, Inc.
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Patent Jurisdiction
United States Patent and Trademark Office
United States Patent and Trademark Office
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Patent Number
76205000
Patent Inventor Names
Claes Gustafsson0
Emily C. Mundorff0
Jeremy S. Minshull0
Sridhar Govindarajan0
Date of Patent
November 17, 2009
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Patent Application Number
103869030
Date Filed
March 10, 2003
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Patent Citations Received
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US Patent 12129495 Engineered DNA polymerase variants
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US Patent 12018295 Engineered DNA polymerase variants
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US Patent 12071642 Engineered amylase variants
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US Patent 12084697 Penicillin-G acylases
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US Patent 12110493 Engineered purine nucleoside phosphorylase variant enzymes
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US Patent 12110521 Engineered methionine gamma lyase variants
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US Patent 12110513 Engineered pantothenate kinase variant enzymes
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US Patent 12129494 T7 RNA polymerase variants
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Patent Primary Examiner
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John S. Brusca
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Patent abstract

Methods and devices for more efficiently engineering diversity into recombinant polypeptides and/or nucleic acids are provided herein. For example, a variety of methods of selecting and/or assessing potential crossover sites in an amino acid sequence or a nucleotide sequence are provided, as well as the resulting chimeric product sequences. These methods include, e.g., consideration of structural, functional and/or statistical data in the selection and assessment of sequences and crossover sites for use in recombination.

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