US Patent 7157242 Method for staining fungi and protozoa

Patent 7157242 was granted and assigned to University of South Florida on January, 2007 by the United States Patent and Trademark Office.

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The present invention provides a method for the staining of fungi and microsporidia for observation with a light microscope based upon the presence of chitin in the composition of these organisms. With the method of the present invention a sample to be analyzed is treated with a solution of Ponceau S and Stains-all dye. The sample is then selectively decolorized and rinsed. The resulting sample is examined with a light microscope, or photographed for a permanent record, to identify the presence of a variety of microorganisms, to include fungi and microsporidia.

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https://pdfpiw.uspto.gov/.piw?Docid=071572420

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