US Patent 7157242 Method for staining fungi and protozoa

Patent 7157242 was granted and assigned to University of South Florida on January, 2007 by the United States Patent and Trademark Office.

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Is a

Patent

Patent attributes

Current Assignee

University of South Florida

Patent Jurisdiction

United States Patent and Trademark Office

Patent Number

71572420

Patent Inventor Names

My Lien Dao0

Date of Patent

January 2, 2007

Patent Application Number

102496630

Date Filed

April 29, 2003

Patent Primary Examiner

‌

Irene Marx

Patent abstract

The present invention provides a method for the staining of fungi and microsporidia for observation with a light microscope based upon the presence of chitin in the composition of these organisms. With the method of the present invention a sample to be analyzed is treated with a solution of Ponceau S and Stains-all dye. The sample is then selectively decolorized and rinsed. The resulting sample is examined with a light microscope, or photographed for a permanent record, to identify the presence of a variety of microorganisms, to include fungi and microsporidia.