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US Patent 11709136 System and method for inferring protein binding

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Is a
Patent
Patent
1

Patent attributes

Patent Applicant
Calico (company)
Calico (company)
1
Current Assignee
Calico (company)
Calico (company)
1
Patent Jurisdiction
United States Patent and Trademark Office
United States Patent and Trademark Office
1
Patent Number
117091361
Date of Patent
July 25, 2023
1
Patent Application Number
172534441
Date Filed
June 17, 2019
1
Patent Citations
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US Patent 10386300 Spectrally and spatially multiplexed fluorescent probes for in situ cell labeling
1
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US Patent 10119912 Apparatus for use in luminescence applications
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US Patent 7456954 Modulated excitation fluorescence analysis
1
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US Patent 7842491 Determination of metal ions in solution by photoluminescence anisotropy
1
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US Patent 8063386 Time resolved fluorescent imaging system
1
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US Patent 8309346 Apparatus for the non-invasive measurement of tissue function and metabolism by determination of steady-state fluorescence anisotropy
1
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US Patent 8948539 System and method for image improvement and enhancement
1
Patent Primary Examiner
‌
David P Porta
1
CPC Code
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G01N 21/6408
1
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G01N 21/6445
1

Methods and apparatus for inferring protein binding based on rotational diffusion of a collection of fluorophores. One example of a method includes applying a first light pulse to excite a plurality of fluorophores in the collection of fluorophores to produce a plurality of excited fluorophores, the first light pulse having a first polarization and the plurality of excited fluorophores having a component of their orientation aligned with the first polarization, applying a second light pulse to stimulate emission by the plurality of excited fluorophores, the second light pulse having a second polarization orthogonal to the first polarization, after a time delay following application of the second light pulse, applying a third light pulse of the second polarization to further stimulate emission by the plurality of excited fluorophores, detecting polarized emissions from the plurality of excited fluorophores, and inferring the rate of rotational diffusion of the collection of fluorophores based on the detected polarized emissions.

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