US Patent 10890580 Reversible cell labelling with conjugates having two releasable binding sites

The invention is directed to a Method for detecting a target moiety in a sample of biological specimens by:a) providing at least one conjugate with the general formula (I)An-P-Bm-Cq-Xo  (I) withA: antigen recognizing moiety;P: enzymatically degradable spacer;B: first binding moietyC second binding moietyX: detection moiety;n, m, q, o integers between 1 and 100,wherein B and C are non-covalently bound to each other and A and B are covalently bound to Pb) labelling the target moiety recognized by the antigen recognizing moiety A with at least one conjugatec) detecting the labelled target moiety via detecting moiety Xd) cleaving Cq-Xo by disrupting the non-covalent bond between Bm and Cq from the labelled target moietye) cleaving the binding moiety Bm from the labelled target moiety by enzymatically degrading spacer P.