US Patent 10197561 Cell detection with conjugates having an enzymatically releasable detection moiety

The invention is directed to a method for detecting a target moiety in a sample of biological specimens by:a) providing at least one conjugate with the general formula (I) Xn—P—Ym, with X is an detection moiety, P an enzymatically degradable spacer and Y an antigen recognizing moiety and n, m are integers between 1 and 100 and wherein X and Y are covalently bound to P;b) contacting the sample of biological specimens with at least one conjugate, thereby labeling the target moiety recognized by the antigen recognizing moiety Y;c) detecting the target moiety labeled with the conjugate with the detecting moiety X; andd) enzymatically degrading spacer P, thereby cleaving the detection moiety X from the conjugate.