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US Patent 10179911 Negative selection and stringency modulation in continuous evolution systems

Patent 10179911 was granted and assigned to President and Fellows of Harvard College on January, 2019 by the United States Patent and Trademark Office.

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Patent attributes

Patent Applicant

President and Fellows of Harvard College

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Current Assignee

President and Fellows of Harvard College

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Patent Jurisdiction

United States Patent and Trademark Office

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Patent Number

Patent Inventor Names

Ahmed Hussein Badran1

David R. Liu1

Jacob Charles Carlson1

Kevin Michael Esvelt1

Date of Patent

January 15, 2019

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Patent Application Number

151127591

Date Filed

January 20, 2015

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Patent Citations Received

US Patent 12084663 Incorporation of unnatural amino acids into proteins using base editing

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US Patent 11912985 Methods and compositions for simultaneous editing of both strands of a target double-stranded nucleotide sequence

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US Patent 11920181 Nuclease profiling system

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US Patent 11932884 High efficiency base editors comprising Gam

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US Patent 11999947 Adenosine nucleobase editors and uses thereof

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US Patent 12006520 Evaluation and improvement of nuclease cleavage specificity

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US Patent 12060553 Evolution of BoNT peptidases

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Patent Primary Examiner

Jeremy C Flinders

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Patent abstract

Strategies, systems, methods, reagents, and kits for phage-assisted continuous evolution are provided herein. These include strategies, systems, methods, reagents, and kits allowing for stringency modulation to evolve weakly active or inactive biomolecule variants, negative selection of undesired properties, and/or positive selection of desired properties.

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