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US Patent 12006520 Evaluation and improvement of nuclease cleavage specificity

Patent 12006520 was granted and assigned to President and Fellows of Harvard College on June, 2024 by the United States Patent and Trademark Office.

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Patent
Patent
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Patent attributes

Patent Applicant
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President and Fellows of Harvard College
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Current Assignee
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President and Fellows of Harvard College
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Patent Jurisdiction
United States Patent and Trademark Office
United States Patent and Trademark Office
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Patent Number
120065200
Patent Inventor Names
David R. Liu0
Vikram Pattanayak0
John Paul Guilinger0
Date of Patent
June 11, 2024
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Patent Application Number
164417510
Date Filed
June 14, 2019
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Patent Citations
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US Patent 7070928 Evolving new molecular function
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US Patent 7078208 Thermostable reverse transcriptases and uses thereof
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US Patent 7083970 Methods and compositions for the production of orthogonal tRNA-aminoacyl tRNA synthetase pairs
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US Patent 7163824 Regulation of endogenous gene expression in cells using zinc finger proteins
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US Patent 7192739 Ligand-dependent protein splicing
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US Patent 7223545 Evolving new molecular function
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US Patent 7354761 Methods and composition for the production of orthogonal tRNA-aminoacyltRNA synthetase pairs
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US Patent 7368275 In vivo incorporation of unnatural amino acids
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...
Patent Primary Examiner
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Frank W. Lu
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Patent abstract

Engineered nucleases are promising tools for genome manipulation and determining off-target cleavage sites of these enzymes is of great interest. This disclosure provides in vitro selection methods that interrogate 10

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