SBIR/STTR Award attributes
Over one million Americans andmillion people worldwide are infected with HIVmaking it one of the most pressing public health concerns of our timeUnfortunatelythere are no vaccines available and treatment requires lifelong anti retroviral therapiesThis proposal combines two technologies for the development of a vaccine against HIVEnva novel adjuvantspontaneous nanoliposome antigen particleizationSNAPanda state of the art antigennative flexibly linkedNFLtrimers of HIVEnve gBGThis proposal will accomplish the following technical objectivesProduce and provide well characterized cobalt porphyrin phosphoipidCoPoPCoPoP liposomes and NFL trimersAssess particleization parameters of His tagged BGNFL trimers with CoPoP liposomesCharacterize conformational stability of particleized NFL trimersDetermine the impact of antigen density in inducing HIV neutralization with rabbit SNAP immunizationAssess multiplexed particleization parameters with His tagged BGandcAssess multivalent IgG response following rabbit SNAP immunization with BGandcandCharacterize cobalt levels and His tag response in immunized subjectsRabbits will be vaccinated and sera tested for neutralizing antibodies to HIVThe neutralizing titer of SNAP NFL vaccinated rabbits will be significantly higher than standard vaccination methods
