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Biohybrid robotics (Bio-syncretic robotics)

Biohybrid robotics (Bio-syncretic robotics)

Biohybrid robotics combines engineered artificial structures and living bio-systems.

Biohybrid robotics combines engineered artificial structures and living bio-systems. The main motivation for biohybrid robots is to provide actuation using bio-hybrid actuators. Biohybrid robots may use bacteria or other motile cells. Actuators have also been based on explanted whole-muscle tissues, cardiomyocytes, optogenetically modified cardiomyocytes, insect self-contractile tissues and engineered skeletal muscle.



Proposed applications for biohybrid actuators include miniaturized therapeutic robots and biohybrid medical devices, in vitro muscle models for drug testing and platforms for investigating muscle contractions. In the longer term biohybrid actuators have applications in the interaction of soft robotic artifacts with humans, microscale devices that perform medical procedures, biobased surveillance systems, manufacturing systems that self-assemble and self-repair and environmental monitoring with swarm biorobots.

Medical applications and microorganism-based biohybrid robots

Biobots that could be injected into a patient’s bloodstream and destroy a blood clot or cancer would require sensing, computation and actuation. Living cells have an advantage over synthetic microswimmers in their ability to detect and respond to environmental stimuli without additional components. Bacteria and algae can be steered using their attraction to light, which is not possible in deep tissues of the body. Magnetic control is one possibility for medical applications since magnetic fields can safely penetrate the body. Magnetotactic bacteria naturally have magnetic nanocrystals and other living cells could be artificially magnetized if embedded with iron oxide nanoparticles or by attaching them to magnetic substrates. Chemicals can also control cell behavior. Biohybrid devices could respond to biochemical signals released from tumor cells. Thermotaxis and aerotaxis are also possible ways to steer microorganism-based biohybrid robots. 



Researchers at Polytechnique Montréal demonstrated magnetic guiding of a magnetotactic bacteria bound to drug-containing nanoliposomes. These Magentococcus marinus bacteria also use aerotaxis, to migrate towards low oxygen in their natural environment. Since tumors are also low oxygen environments, the researchers demonstrated in mice that this aerotaxis can be exploited for delivering drugs to tumors. 

Biohybrid soft robotics

Beating cells from a rat (ventricular cardiomyocytes) were first placed on poly(dimethylsiloxane) (PDMS) thin films in 2007 by Whitesides and Parker. This work defined a biohybrid soft robotic device as a hybrid device which has a soft body and where the actuation is performed by a living biological part. Soft biohybrid robots go beyond replication of nature, but seek to fulfill tasks that neither nature nor physical robots can achieve. 



Professors Anne Staubitz and Christine Selhuber-Unkel at University of Bremen and University of Kiel proposed a taxonomic scheme for the concept of “living materials” used in biohybrid soft robotics. Their taxonomy builds on the taxonomy scheme put forth by Webster-Wood. 



The following are examples of biohybrid soft robots described by the taxonomy scheme. The walker is a biobot actuated by a skeletal muscle ring modified for optogenetic control with light. The pump is a bio-micropump on a chip powered by earthworm muscle.





Neuromuscular tissue

Researchers at University of Illinois developed soft robotic devices driven by neuromuscular tissue that triggers when stimulated by light using optogenetics, which was published in 2019 in PNAS. In 2014, the team developed self-propelled biohybrid swimming and walking biobots that uses cardiac muscle cells from rats, which beat on their own, as motors. Their swimming biobots were modeled after sperm, with a single tail. In the 2019 version, optogenetic neuron cells, derived from mouse stem cells, were added to their device with two tails. The biobots self assemble since neurons advanced towards the muscle and formed neuromuscular junctions. The resulting neuromuscular tissue worked with their synthetic biobot skeletons. Muscle activity is controlled by neuron activity, which is controlled by exposure to light using optogenetics.

Timeline

March 30, 2018

Electrically Driven Microengineered Bio-inspired Soft Robots

Su Ryon Shin and Ali Khademhosseini are corresponding authors

Harvard Medical School, King Abdulaziz University, Jeddah, Saudi Arabia and Konkuk University, Seoul

An autonomous structure-controlled self-actuating ray powered by cardiomyocytes using design and structuring principles.

June 28, 2017

Light controlled 3D micromotors powered by bacteria

Gaszton Vizsnyiczai, Giacomo Frangipane, Claudio Maggi, Filippo Saglimbeni, Silvio Bianchi & Roberto Di Leonardo

NANOTEC-CNR, Institute of Nanotechnology, Soft and Living Matter Laboratory, Rome

"The synthetic components consist of 3D interconnected structures having a rotating unit that can capture individual bacteria into an array of microchambers so that cells contribute maximally to the applied torque. Bacterial cells are smooth swimmers expressing a light-driven proton pump that allows to optically control their swimming speed."

July 8, 2016

Phototactic guidance of a tissue-engineered soft-robotic ray

Park, Sung-Jin et al. Corresponding author Kevin Kit Parker.

Harvard University, Sogang University, Stanford University

Swimming robot mimics a ray fish (1/10 scale version) powered by cardiomyocytes genetically engineered to respond to light cues. The robot was built with microfabricated gold skeleton and rubber body.

July 15, 2014

Three-dimensionally printed biological machines powered by skeletal muscle

Caroline Cvetkovic, Ritu Raman, Vincent Chan, Brian J. Williams, Madeline Tolish, Piyush Bajaj, Mahmut Selman Sakar, H. Harry Asada, M. Taher A. Saif, and Rashid Bashir

University of Illinois at Urbana-Champaign, MIT

July 11, 2012

Room Temperature Operable Autonomously Moving Bio-Microrobot Powered by Insect Dorsal Vessel Tissue

Yoshitake Akiyama,Takayuki Hoshino,Kikuo Iwabuchi,Keisuke Morishima

Osaka University, Tokyo University of Agriculture and Technology

September 2007

A micro-spherical heart pump powered by cultured cardiomyocytes

Yo Tanaka, Kae Sato, Tatsuya Shimizu, Masayuki Yamato, Teruo Okanobd and Takehiko Kitamori

The University of Tokyo, Japan Science and Technology Agency, Kanagawa Academy of Science and Technology (KAST)

February 2007

Muscular Thin Films for Building Actuators and Powering Devices

Adam W. Feinberg, Alex Feigel, Sergey S. Shevkoplyas, Sean Sheehy, George M. Whitesides*, Kevin Kit Parker*

Harvard University

Cell sheet engineering using rat cardiomyocytes on polydimethylsiloxane thin films was used to build centimeter-scale biohybrid constructs that perform functions as diverse as gripping, pumping, walking, and swimming with fine spatial and temporal control.

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Development and Future Challenges of Bio-Syncretic Robots



Web



Living Materials Herald a New Era in Soft Robotics

Clement Appiah, Christine Arndt, Katharina Siemsen, Anne Heitmann, Anne Staubitz, Christine Selhuber-Unkel

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July 3, 2019

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