In various embodiments, a staple cartridge for use with a surgical stapler is disclosed. The staple cartridge comprises a cartridge body comprising a deck, a row of first staple cavities defined in the deck, a plurality of first staples, wherein the first staples are positioned in the first staple cavities, a row of second staple cavities defined in the deck, and a plurality of second staples, wherein the second staples are positioned in the second staple cavities. The staple cartridge further comprises a first layer comprising a first portion positioned over the first staple cavities and configured to be captured within the first staples and a second portion positioned over the second staple cavities and configured to be captured within the second staples. The staple cartridge further comprises a second layer positioned over the second portion and not positioned over the first portion of the first layer.