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US Patent 9329174 Bead trapping method and method for detecting target molecule

Patent 9329174 was granted and assigned to Japan Science and Technology Agency on May, 2016 by the United States Patent and Trademark Office.

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Patent attributes

Current Assignee
Japan Science and Technology Agency
Japan Science and Technology Agency
Patent Jurisdiction
United States Patent and Trademark Office
United States Patent and Trademark Office
Patent Number
9329174
Date of Patent
May 3, 2016
Patent Application Number
14003509
Date Filed
March 7, 2012
Patent Citations Received
‌
US Patent 12077808 Microfluidic devices, solid supports for reagents and related methods
3
‌
US Patent 11835460 System and method with fiducials having offset layouts
4
Patent Primary Examiner
‌
Maria Leavitt
Patent abstract

This invention provides a technique enabling to detect target molecules of low concentration with high sensitivity. This invention includes (i) a step of introducing a hydrophilic solvent (42) containing beads (40),(41′) into a space (30) between (a) a lower layer section (10) including a plurality of receptacles (13) each of which is capable of storing only one of the beads (41),(41′) and which are separated from each other by a side wall (12) having a hydrophobic upper surface and (b) an upper layer section (20) facing a surface of the lower layer section (10) on which surface the plurality of receptacles (13) are provided; and (ii) a step of introducing a hydrophobic solvent (43) into the space (30), the step (ii) being carried out after the step (i).

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