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US Patent 9267127 Evolution of bond-forming enzymes

Patent 9267127 was granted and assigned to President and Fellows of Harvard College on February, 2016 by the United States Patent and Trademark Office.

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Patent attributes

Patent Applicant

President and Fellows of Harvard College

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Current Assignee

President and Fellows of Harvard College

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Patent Jurisdiction

United States Patent and Trademark Office

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Patent Number

Patent Inventor Names

Irwin Chen1

Brent M. Dorr1

David R. Liu1

Date of Patent

February 23, 2016

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Patent Application Number

139228121

Date Filed

June 20, 2013

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Patent Citations Received

US Patent 12084663 Incorporation of unnatural amino acids into proteins using base editing

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US Patent 11999947 Adenosine nucleobase editors and uses thereof

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US Patent 12006520 Evaluation and improvement of nuclease cleavage specificity

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US Patent 12060553 Evolution of BoNT peptidases

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US Patent 12077795 Method for biocatalytic protein-oligonucleotide conjugation

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US Patent 11702651 Adenosine nucleobase editors and uses thereof

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Patent Primary Examiner

1

Patent abstract

Strategies, systems, methods, reagents, and kits for the directed evolution of bond-forming enzymes are provided herein. Evolution products, for example, evolved sortases exhibiting enhanced reaction kinetics and/or altered substrate preferences are also provided herein, as are methods for using such evolved bond-forming enzymes. Kits comprising materials, reagents, and cells for carrying out the directed evolution methods described herein are also provided.

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