US Patent 9163246 T7 expression system
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Patent abstract
The present invention provides an improved prokaryotic cell expression system employing a tightly controlled host strain construct that controls uninduced, leaky expression of proteins while still auto-inducing well. Various aspects of the present invention address and overcome the problem of uninduced basal expression by providing a host strain that comprises a T7 polymerase gene, and mutants thereof, inserted between lac Z and lac Y of the lac operon (a “ZRY” construct), downstream of an otherwise wild-type lac operon control region.
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US Patent 09163246 T7 expression system US Patent 9163246 T7 expression system
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Patent Number
0916324600
Patent Number
916324600
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Patent Jurisdiction
Patent Number
091632460
Date of Patent
October 20, 2015
0Patent Application Number
126726060
Date Filed
August 8, 2008
0Official Website
Patent Primary Examiner
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Patent Jurisdiction
Patent Number
091632460
Date of Patent
October 20, 2015
0Patent Application Number
126726060
Date Filed
August 8, 2008
0Official Website
Patent Primary Examiner
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US Patent 09163246 T7 expression system
Infobox
Is a
Patent jurisdiction
Patent number
09163246
Date of patent
October 20, 2015
Patent application number
12672606
Date Filed
August 8, 2008
Patent primary examiner
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