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US Patent 8993341 Removal of PCR inhibitors

Patent 8993341 was granted and assigned to Exact Sciences (company) on March, 2015 by the United States Patent and Trademark Office.

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Patent
Patent
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Patent attributes

Current Assignee
Exact Sciences (company)
Exact Sciences (company)
1
Patent Jurisdiction
United States Patent and Trademark Office
United States Patent and Trademark Office
1
Patent Number
89933411
Patent Inventor Names
Michael J. Domanico1
James P. Light, II1
Janelle J. Bruinsma1
Hemanth D. Shenoi1
Date of Patent
March 31, 2015
1
Patent Application Number
134699891
Date Filed
May 11, 2012
1
Patent Citations Received
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US Patent 11987847 Detecting colorectal neoplasm
2
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US Patent 11821039 Detecting neoplasm
3
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US Patent 11859254 Detecting gastric neoplasm
4
Patent Primary Examiner
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Gary Benzion
1
Patent abstract

Provided herein is technology relating to processing and preparing samples and particularly, but not exclusively, to methods, systems, and kits for removing assay inhibitors, e.g, compounds that inhibit polymerase chain reaction, from samples comprising nucleic acids. In particular, the technology is directed toward treating crude sample preparations, such as supernatants from homogenized stool samples, with insoluble polyvinylpyrrolidone (PVP) to form PVP-assay inhibitor complexes, and spin filtration to separate the PVP-assay inhibitor complexes from the crude sample preparations to produce clarified samples that exhibit reduced assay inhibition.

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