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US Patent 8715934 Single-molecule PCR on microparticles in water-in-oil emulsions

Patent 8715934 was granted and assigned to Johns Hopkins University on May, 2014 by the United States Patent and Trademark Office.

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Patent
Patent

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Current Assignee
Johns Hopkins University
Johns Hopkins University
Patent Jurisdiction
United States Patent and Trademark Office
United States Patent and Trademark Office
Patent Number
8715934
Date of Patent
May 6, 2014
Patent Application Number
12305825
Date Filed
June 19, 2007
Patent Citations Received
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US Patent 12091710 Systems and methods for handling microfluidic droplets
0
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US Patent 11866782 Multi-omic analysis in monodisperse droplets
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US Patent 11904322 Methods and systems for nucleic acid analysis
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US Patent 11965877 Compositions and methods for molecular labeling
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US Patent 11747327 Compositions and methods for molecular labeling
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US Patent 11754499 Enzyme quantification
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US Patent 11768198 Compositions and methods for molecular labeling
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US Patent 11773452 Single cell sequencing
...
Patent Primary Examiner
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Robert T. Crow
Patent abstract

Modulation of the viscosity of the oil phase of a microemulsion used for amplification of DNA on a bead increases the homogeneity of product beads and the amount of amplified DNA per bead. Moreover the number of separate microemulsion populations that can be formed in parallel is increased using multi-well plates and mixer mill disruptor machines designed to lyse biological samples.

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