US Patent 8351675 Systems and methods for volumetric tissue scanning microscopy
Patent 8351675 was granted and assigned to Massachusetts Institute of Technology on January, 2013 by the United States Patent and Trademark Office.
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In accordance with preferred embodiments of the present invention, a method for imaging tissue, for example, includes the steps of mounting the tissue on a computer controlled stage of a microscope, determining volumetric imaging parameters, directing at least two photons into a region of interest, scanning the region of interest across a portion of the tissue, imaging a plurality of layers of the tissue in a plurality of volumes of the tissue in the region of interest, sectioning the portion of the tissue and imaging a second plurality of layers of the tissue in a second plurality of volumes of the tissue in the region of interest, detecting a fluorescence image of the tissue due to said excitation light; and processing three-dimensional data that is collected to create a three-dimensional image of the region of interest.
US Patent 08351675 Systems and methods for volumetric tissue scanning microscopy US Patent 8351675 Systems and methods for volumetric tissue scanning microscopy
US Patent 08351675 Systems and methods for volumetric tissue scanning microscopy
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