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US Patent 8119381 Rationally-designed meganucleases with altered sequence specificity and DNA-binding affinity

Patent 8119381 was granted and assigned to Duke University on February, 2012 by the United States Patent and Trademark Office.

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Patent abstract

Rationally-designed LAGLIDADG meganucleases and methods of making such meganucleases are provided. In addition, methods are provided for using the meganucleases to generate recombinant cells and organisms having a desired DNA sequence inserted into a limited number of loci within the genome, as well as methods of gene therapy, for treatment of pathogenic infections, and for in vitro applications in diagnostics and research.

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