US Patent 7998694 Method of revealing a biological process using a FRET measurement

The present invention relates to a method of revealing a biological process using a FRET measurement, which comprises the following steps:incorporating, into a measurement medium containing a lipid membrane, a biological entity X coupled with a first member of a pair of FRET partners and a second biological entity Y coupled with the second member of the pair of FRET partners, the energy-donating member of the pair of FRET partners having a long lifetime and the members of said pair of FRET partners being located on either side of the lipid membrane;exciting the measurement medium at the excitation wavelength of the energy-donating member; andmeasuring the FRET signal or the variations in said signal emitted in said culture medium.