A sleepiness level detection method of determining a sleepiness level of a subject, includes: detecting a heart beat signal indicating a signal of heart beats from the subject and storing the heart beat signal in a storage device; detecting amplitude peaks of the heart beat signal and detecting an interval between the amplitude peaks as a heart beat interval; and computing a spectral density corresponding to fluctuation in the heart beat interval detected and determining the sleepiness level based on a maximum frequency at which the spectral density is maximum.