US Patent 7955867 High throughput cell-based assays, methods of use and kits
Patent 7955867 was granted and assigned to Millipore Sigma on June, 2011 by the United States Patent and Trademark Office.
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In the present invention cells are placed in a multiwell plate and grown. When the assay is to be performed, one uses gravity to wash away any unbound ligands rather than vacuum or centrifugation. The cells are then examined to detect the bound ligand. To perform the washing step(s) the plate is placed into a carrier plate having open wells in register with the wells of the filter plate or one may use a wicking device or an underdrain attached to the bottom of the filter plate. Sufficient wash liquid is added to allow for filtration by the effect of gravity to occur. Cells are retained within the wells at a rate of 4 times that of other rapid methods.
US Patent 07955867 High throughput cell-based assays, methods of use and kits US Patent 7955867 High throughput cell-based assays, methods of use and kits
US Patent 07955867 High throughput cell-based assays, methods of use and kits
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