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US Patent 7939296 Method for production of recombinant human FSH

Patent 7939296 was granted and assigned to JCR Pharmaceuticals on May, 2011 by the United States Patent and Trademark Office.

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Is a

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Patent attributes

Current Assignee

JCR Pharmaceuticals

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Patent Jurisdiction

United States Patent and Trademark Office

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Patent Number

Patent Inventor Names

Atsushi Sugimura0

Yae Ito0

Kazutoshi Mihara0

Katsuya Daimon0

Date of Patent

May 10, 2011

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Patent Application Number

124666520

Date Filed

May 15, 2009

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Patent Primary Examiner

Tekchand Saidha

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Patent abstract

Disclosed is a method for production of recombinant human FSH in high yield and a high purity. The method comprises the steps of: (a) culturing recombinant human FSH-producing mammalian cells in a serum-free medium, (b) collecting culture supernatant, (c) subjecting the culture supernatant to cation-exchange column chromatography, (d) dye affinity column chromatography, (e) hydrophobic column chromatography, and (f) gel filtration column chromatography to collect recombinant human FSH-active fractions, in the order.

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