This invention relates to Pulsatillae Radix extract for improving brain functions. More specifically, this invention relates to Pulsatillae Radix extract, its active fractions and a pharmaceutical product and a health food containing the same respectively having a protective activity against neurotoxicity and a growth inhibitory effect induced by beta-amyloid, an anti-oxidizing effect, a neuron proliferating effect and improving memory thereby effective in improving brain functions such as Mild Cognitive Impairment and dementia.