A centrifugal fraction collection system including a rotating carrier for holding at least one sample collection container and inducing a centrifugal force, a guide disposed on the rotating carrier, and a flexible eluant tube disposed over the rotating carrier and through the guide for receiving a flow of eluant having volatile and non-volatile components and directing the flow of eluant into at least one sample collection container wherein the centrifugal force separates the non-volatile and volatile components based on their respective densities and collects the non-volatile components in at least one sample collection container.