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US Patent 7871777 Probe for nucleic acid sequencing and methods of use

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Is a
Patent
Patent

Patent attributes

Patent Jurisdiction
United States Patent and Trademark Office
United States Patent and Trademark Office
Patent Number
7871777
Date of Patent
January 18, 2011
Patent Application Number
12095973
Date Filed
December 12, 2006
Patent Citations Received
‌
US Patent 12123834 Active-source-pixel, integrated device for rapid analysis of biological and chemical specimens
3
‌
US Patent 12078596 Hand-held, massively-parallel, bio-optoelectronic instrument
4
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US Patent 12111261 Integrated photodetector with direct binning pixel
5
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US Patent 12123772 Integrated photodetector with charge storage bin of varied detection time
6
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US Patent 11674174 Nucleic acid sequences using tags
7
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US Patent 11879841 Optical system and assay chip for probing, detecting and analyzing molecules
8
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US Patent 11959853 Integrated device with external light source for probing, detecting and analyzing molecules
9
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US Patent 11970729 Methods for nucleic acid sequencing
10
Patent Primary Examiner
‌
Ethan Whisenant
Patent abstract

A nanoprobe for sequencing of nucleic acid molecules is provided, as well as methods for using the nanoprobe. In particular examples, the probe includes a polymerizing agent and one or more molecular linkers that carry a chemical moiety capable of reversibly binding to the template strand of a nucleic acid molecule, without being detached from the linker, by specifically binding with a complementary nucleotide in the target nucleic acid molecule. The reversible binding of the chemical moiety on the linker with a complementary nucleotide in the target nucleic acid molecule is indicated by emission of a characteristic signal that indicates pairing of the chemical moiety on the linker with its complementary nucleotide. An example of such a chemical moiety is a nonhydrolyzable nucleotide analog. In particular examples, the polymerizing agent and the chemical moiety are associated with a tag, such as a donor fluorophore and acceptor fluorophore characteristic of the particular type of chemical moiety.

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