Is a
Patent attributes
Current Assignee
Patent Jurisdiction
Patent Number
Patent Inventor Names
Brian J. Reardon0
Susan H. Julien0
Hong Y. Liu0
Chung-leung Chan0
Date of Patent
December 28, 2010
0Patent Application Number
113013830
Date Filed
December 12, 2005
0Patent Primary Examiner
Patent abstract
The expression vectors and methods using an E. coli expression system for the large scale production of FGF18 are described. The vectors utilize the FGF18 coding sequence with specific changes in nucleotides in order to optimize codons and mRNA secondary structure for translation in E. coli. Using the expression vectors, the FGF18 gene was produced in E. coli to a level of greater than 1 g/L in fed batch fermentation. Also included are OmpT deficient E. coli strains, as well as OmpT and fhuA negative strains transformed with an FGF18 expression vector.
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