Is a
Patent attributes
Current Assignee
Patent Jurisdiction
Patent Number
Patent Inventor Names
James A. Ernst0
Daniel Yansura0
Hok Seon Kim0
Date of Patent
November 9, 2010
Patent Application Number
12541828
Date Filed
August 14, 2009
Patent Primary Examiner
Patent abstract
Methods for producing membrane-spanning polypeptides in high yields, with native conformation, and/or in soluble form include solubilizing in non-ionic or zwitterionic detergents, as well as use of promoters and expression vectors for expressing high yields of membrane-spanning polypeptides in bacterial cells. Mutated promoters provide tight control of membrane-spanning polypeptides in bacterial cell hosts.
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