Is a
Patent attributes
Patent Jurisdiction
Patent Number
Date of Patent
February 16, 2010
Patent Application Number
10182996
Date Filed
February 1, 2001
Patent Citations Received
Patent Primary Examiner
Patent abstract
Disclosed is a method for increasing the fluorescence of a Cyanine dye molecule comprising at least one NO2 group characterized by the reduction of the at least one NO2 group to NHOH or NH2 by the action of a nitroreductase. The cyanine dye molecule comprising at least one NO2 group can be used as a substrate for detecting nitroreductase enzyme activity in a composition and allows for the use of a nitroreductase enzyme in an enzyme-reporter system for the detection of analytes, binding reactions or gene expression.
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