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US Patent 7638276 In vitro sorting method

Patent 7638276 was granted and assigned to 454 Life Sciences on December, 2009 by the United States Patent and Trademark Office.

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Patent

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Current Assignee
454 Life Sciences
454 Life Sciences
Patent Jurisdiction
United States Patent and Trademark Office
United States Patent and Trademark Office
Patent Number
7638276
Date of Patent
December 29, 2009
Patent Application Number
10861930
Date Filed
June 4, 2004
Patent Citations Received
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US Patent 12131805 Sequencing methods
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US Patent 12110549 Methods and systems for processing polynucleotides
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US Patent 12116631 Creation of libraries of droplets and related species
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US Patent 12121898 Droplet creation techniques
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US Patent 11667954 Method and apparatus to normalize quantitative readouts in single-cell experiments
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US Patent 11697841 Development of novel detergents for use in PCR systems
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US Patent 11713457 Methods and systems for processing polynucleotides
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US Patent 11747327 Compositions and methods for molecular labeling
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Patent Primary Examiner
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Robert M Kelly
Patent abstract

The invention describes a method for isolating one or more genetic elements encoding a gene product having a desired activity, comprising of the steps of: (a) compartmentalising genetic elements into microcapsules; (b) expressing the genetic elements to produce their respective gene products within the microcapsules; (c) sorting the genetic elements which produce the gene product having a desired activity. The invention enables the in vitro evolution of nucleic acids by repeated mutagenesis and iterative applications of the method of the invention.

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