US Patent 7585400 Chip for electrochemical immunoassay

The present invention provides a chip 100 that enables an immunoassay with high accuracy and without requiring a high volatile reagent. The chip 100 includes a chip substrate 20, a reagent mixture 12 and potassium ferricyanide 11 that are immobilized leaving a space in between on the chip substrate 20. The reagent mixture 12 includes at least one selected from NADP and NADPH, malate dehydrogenase, and a substrate of malate dehydrogenase. The chip 100 further includes diaphorase, immobilized on the chip substrate 20, as a part of or a component other than the reagent mixture 12.