A method of supporting denitrification includes receiving biological material at a pulsed electric field station and applying a pulsed electric field to the biological material within a treatment zone in the pulse electric field station to generate treated biological material. The method also includes transporting at least a portion of the treated biological material to an anoxic bioreactor in substitution, at least in part, for an external source of electron donor. A system for supporting denitrification is also provided.