A method for improving a reading resolution of a biosensor is provided. The present invention includes applying a specimen on a chip of a biosensor, and a voltage-time discharge curve is established in response to a specific component in the specimen detected by the chip. Using a voltage V0 of a sampling time t0 of the voltage-time discharge curve as a central voltage, and selecting a plurality of respective voltages of sampling times close to the sampling time t0. Obtaining an average voltage of the central voltage V0 and these selected respective voltages to serve as an output voltage of the sampling time t0. Determining a respective average voltage corresponding to each sampling time of the voltage-time discharge curve before the time of discharge ending based on the former step, to serve as the output voltage of the each sampling time. The each output voltage is converted to a set of digital signals. A reading of the concentration of the specific component in the specimen is determined in accordance with these digital signals.