US Patent 7498166 Dual expression vector system and screening methods
Patent 7498166 was granted and assigned to Vertex Pharmaceuticals on March, 2009 by the United States Patent and Trademark Office.
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The present invention features vectors that contain a promoter effective for expression in bacterial cells and a promoter effective for expression in insect cells. The dual promoter system allows use of the same vector in both host cell systems so that construction of only a single vector is needed to express a polynucleotide inserted at a downstream cloning site. In preferred embodiments the vector is used to derive a recombinant baculovirus that is used to infect host cells. In particular vectors the promoters are a baculovirus polh promoter and a T7lac promoter. In particular vectors the promoter effective for expression in bacteria is positioned between the promoter effective for expression in insect cells and a cloning site. The invention also features various high throughput screening methods.
US Patent 07498166 Dual expression vector system and screening methods US Patent 7498166 Dual expression vector system and screening methods
US Patent 07498166 Dual expression vector system and screening methods
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