Is a
Patent attributes
Current Assignee
Patent Jurisdiction
Patent Number
Date of Patent
November 25, 2008
Patent Application Number
11034848
Date Filed
January 14, 2005
Patent Primary Examiner
Patent abstract
Raffinose is produced by allowing a raffinose synthase having the following properties to act on sucrose and galactinol:(1) action and substrate specificity: produces raffinose from sucrose and galactinol;(2) optimum pH: about 6 to 8;(3) optimum temperature: about 35 to 40° C.;(4) molecular weight:(i) about 75 kDa to 95 kDa estimated by gel filtration chromatography;(ii) about 90 kDa to 100 kDa estimated by polyacrylamide gel electrophoresis (Native PAGE); and(iii) about 90 kDa to 100 kDa estimated by SDS-polyacrylamide gel electrophoresis under a reduced condition (SDS-PAGE); and(5) inhibition: inhibited by iodoacetamide, N-ethylmaleimide, and myo-inositol.
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