Is a
Patent attributes
Current Assignee
Patent Jurisdiction
Patent Number
Patent Inventor Names
Makoto Ueda0
Nobuyoshi Esaki0
Hisaaki Mihara0
Mari Hara0
Date of Patent
November 18, 2008
0Patent Application Number
114383160
Date Filed
May 23, 2006
0Patent Citations Received
Patent Primary Examiner
Patent abstract
The object of the present invention is to provide a novel dehydrogenase having a property which is different from that of known dehydrogenases. The present invention provides a dehydrogenase having the following physicochemical properties:(1) effect: to produce N-alkyl-L-alanine from pyruvic acid and alkylamine or dialkylamine using NADPH and/or NADH as coenzyme;(2) substrate specificity: to show activity to alkylamine or dialkylamine but not to ammonium;(3) optimal pH when using phenylpyruvic acid and methylamine as substrates is around 10; and(4) when treated at 30° C. for 30 minutes, the enzyme is stable at around pH 5 to 10.5.
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