A stand tube of a bicycle comprises a body; one end of the body formed with a U shape ear; each leg of the U shape ear having a pivotal hole; a head having one end formed with a round portion; the round portion having a transversal penetrating hole; a buckling unit having a pivotal unit; a receiving hole penetrating through the buckling unit; the buckling unit passing through the two pivotal holes and the penetrating hole; two buckles; one surface of the buckle being protruded with a protrusion; and a screw unit received in the receiving hole of the pivotal unit; the teeth of two buckles being then engaged to the teeth portion of the U shape ear and the two protrusion being received into the pivotal hole. By above assembly, the stand tube of a bicycle has a rigid and firm structure.