Patent attributes
In one or more embodiments, a microscope has a first pulsed laser and a second pulsed laser arranged to irradiate a specimen. Coherent Raman scattering light emanated from the irradiated specimen is extracted, along with multiphoton excitation fluorescence and a second harmonic wave. One or more detectors detect the extracted coherent Raman scattering light, the extracted fluorescence, and the second harmonic wave. A single specimen can simultaneously be observed with respect to either parallel or selective observations of the two-photon excitation fluorescence, the second harmonic wave, and the coherent Raman scattered light.