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US Patent 7255994 Ligation assay

Patent 7255994 was granted and assigned to Applera on August, 2007 by the United States Patent and Trademark Office.

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Patent
Patent

Patent attributes

Current Assignee
‌
Applera
Patent Jurisdiction
United States Patent and Trademark Office
United States Patent and Trademark Office
Patent Number
7255994
Date of Patent
August 14, 2007
Patent Application Number
10866012
Date Filed
June 10, 2004
Patent Citations Received
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US Patent 12129516 Quantitative and automated permeabilization performance evaluation for spatial transcriptomics
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US Patent 12116626 AP50 polymerases and uses thereof
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US Patent 12117439 Compositions and methods for using fixed biological samples
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US Patent 12128403 Fluid delivery methods
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US Patent 11702698 Enhancing specificity of analyte binding
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US Patent 11702693 Methods for printing cells and generating arrays of barcoded cells
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US Patent 11713485 Hybridization chain reaction methods for in situ molecular detection
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US Patent 11753673 Methods, compositions, and kits for blocking a capture probe on a spatial array
...
Patent Primary Examiner
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Ethan Whisenant
Patent abstract

Disclosed are methods and kits for detecting or quantifying one or more target polynucleotide sequences in a sample. Embodiments of the invention employ a first ligation reaction, a subsequent optional amplification reaction, and a second ligation reaction. Embodiments of the invention combine the specificity of both hybridization and ligation reactions along with universal probe polynucleotide sequences to achieve specific and multiplexed detection of a plurality of target polynucleotide sequences.

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