US Patent 7250274 IL-21 production in prokaryotic hosts

Patent 7250274 was granted and assigned to ZymoGenetics on July, 2007 by the United States Patent and Trademark Office.

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Is a

Patent

Patent attributes

Current Assignee

ZymoGenetics

Patent Jurisdiction

United States Patent and Trademark Office

Patent Number

7250274

Date of Patent

July 31, 2007

Patent Application Number

10735149

Date Filed

December 12, 2003

Patent Primary Examiner

‌

Gary B. Nickol

Patent abstract

The expression vectors and methods using an E. coli expression system for the large scale production of IL-21 are described. The vectors utilize the IL-21 coding sequence with specific changes in nucleotides in order to optimize codons and mRNA secondary structure for translation in E. coli. Using the expression vectors, the IL-21 gene was produced in E. coli to a level of greater than 1 g/L in fed batch fermentation. Also included are OmpT deficient E. coli strains transformed with an IL-21 expression vector.