Patent 7220549 was granted and assigned to Helicos Biosciences on May, 2007 by the United States Patent and Trademark Office.
The invention provides methods for sequencing a nucleic acid comprising stabilizing a primer/target nucleic acid duplex on a substrate. Methods of the invention generally contemplate the use of a dual-anchored primer/target nucleic acid duplex, or a stabilizing molecule in a single molecule sequencing reaction.