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Patent attributes
Current Assignee
0
Patent Jurisdiction
Patent Number
Patent Inventor Names
Arnold H. Horwitz0
Date of Patent
March 20, 2007
0Patent Application Number
104047240
Date Filed
March 31, 2003
0Patent Primary Examiner
Patent abstract
The present invention provides novel methods and materials for increasing the expression of recombinant polypeptides. Methods and materials of the invention allow increased expression of transcription units that include recombinant DNA sequences which encode polypeptides of interest. The present invention provides expression vectors which contain multiple copies of a transcription unit encoding a polypeptide of interest separated by at least one selective marker gene and methods for sequentially transforming or transfecting host cells with expression vectors to increase transcription unit dosage and expression.
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