Aseptic sampling valve having a valve body with a generally cylindrical side wall and a relatively thin, generally planar end wall, an inlet opening in the end wall, a valve seat surrounding the inlet opening, a port opening through the side wall and extending toward the inlet opening at an acute angle relative to the axis of the valve body, and a valve member movable axially within the valve body between open and closed positions relative to the valve seat for controlling communication between the inlet opening and the port.