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US Patent 7188030 Automatic threshold setting for quantitative polymerase chain reaction

Patent 7188030 was granted and assigned to Applera on March, 2007 by the United States Patent and Trademark Office.

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Patent abstract

Disclosed are systems and methods for identifying and quantitating the presence of one or more DNA species in a sample population through PCR amplification. DNA species quantitation includes a determination of a threshold fluorescence value used in the assessment of the PCR amplification reaction. Various embodiments of the present invention incorporate an enhancement function useful in selecting appropriate threshold fluorescence values and facilitate the determination of DNA concentrations by quantitative PCR based methodologies.

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