Patent 7186542 was granted and assigned to Oncolytics Biotech on March, 2007 by the United States Patent and Trademark Office.
The present invention is directed to a method of extracting virus, particularly reovirus, from a culture of cells. Infectious virus can be extracted from the culture with a detergent at a convenient temperature such as 25° C. or 37° C. to produce high virus titers. Both ionic and non-ionic detergents can be used in the present invention.