Patent attributes
The present invention provides a nucleotide having the formula,Sig-PM-SM-BASEwherein PM is a phosphate moiety, SM is a sugar moiety and BASE is a pyrimidine, purine or 7-deazapurine moiety. PM is attached to the 3′ or the 5′ position of the sugar moiety when the nucleotide is a deoxyribonucleotide and at the 2′, 3′ or 5′ position when the nucleotide is a ribonucleotide. BASE is attached to the 1′ position of SM from the N1 position when BASE is a pyrmidine or the N9 position when BASE is a purine or 7-deazapurine. Sig is covalently attached to PM directly or via a chemical linkage, and represents a detectable moiety covalently attached to SM directly or through a linkage group. Also provided are an oligo- or polynucleotide comprising at least one such phosphate-moiety labeled nucleotide, and other compositions including those wherein a polypeptide is terminally ligated or attached to the oligo- or polynucleotide. The phosphate-moiety labeled nucleotide, and the oligo- or polynucleotides and other compositions comprising such phosphate-moiety labeled nucleotides, are useful as diagnostic tools for detecting analytes and as therapeutic agents.
