Apparatuses and methods for connecting and disconnected fluid conduits (e.g. medical tubing) are disclosed. Typical embodiments of the Apparatuses and methods disclosed herein operate with two separate components, the first having a moveable septum and the second having a fixed septum. When the components are engaged, the device allows the flow of fluid through a conduit to which they are coupled. When the components are uncoupled, the device prevents the flow of fluid through the conduit. An exemplary embodiment of the invention includes a housing, a moveable septum slideably coupled with the housing to be alternately disposed in at least a first and second position and a piercing member having a passage for conducting fluid. The moveable septum closes the passage in the first position and is penetrated by the piercing member to open the passage in the second position.