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US Patent 6893850 Method for cell patterning

Patent 6893850 was granted and assigned to President and Fellows of Harvard College on May, 2005 by the United States Patent and Trademark Office.

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Patent abstractTimelineTable: Further ResourcesReferences
Is a
Patent
Patent
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Patent attributes

Patent Applicant
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1
Current Assignee
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President and Fellows of Harvard College
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Patent Jurisdiction
United States Patent and Trademark Office
United States Patent and Trademark Office
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Patent Number
68938501
Patent Inventor Names
Ravi Kane1
David C. Duffy1
Emanuele Ostuni1
George M. Whitesides1
Rebecca J. Jackman1
Date of Patent
May 17, 2005
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Patent Application Number
098087451
Date Filed
March 15, 2001
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Patent Citations Received
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US Patent 12007315 Sample collection and handling for delayed analysis
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US Patent 11927560 Bio/chemical material extraction and assay
3
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US Patent 11885952 Optics, device, and system for assaying and imaging
4
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US Patent 11883824 Assay using different spacing heights
5
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US Patent 12066434 QMAX assays and applications
6
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US Patent 11940382 Assay with amplification
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Patent Primary Examiner
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David M. Haff
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Patent abstract

The present invention provides a masking system for selectively applying cells to predetermined regions of a surface. A mask is positioned adjacent to a surface to cover some portions of the surface while allowing other portions of the surface to remain uncovered. Cells then are applied to uncovered portions of the surface and the mask removed. Alternatively, a cell-adhesion promoter is applied to uncovered portions of the surface, and then cells are applied to the surface before or after removal of the mask from the surface. The masking system can be pre-coated, at least on those surfaces which will come into contact with cells, with a cell-adhesion inhibitor to resist absorption of cells and thereby avoid cell damage when the mask is removed (if cells are deposited prior to removal of the mask). A polymeric elastomeric mask that comes into cohesive-conformal contact with a surface to be patterned can be used.

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